| Product Name | Eotaxin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCYA11, Eotaxin, C-C motif chemokine 11SCYA11 |
| Product description | Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses. |
| Immunogen | Eotaxin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL11 |
|---|---|
| Alternative Names | SCYA11 Eotaxin C-C motif chemokine 11SCYA11 |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Eotaxin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
