Application
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. LaurenApplication
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. LaurenApplication
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. LaurenApplication
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.Application
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with ProSci's biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.| Product Name | Eotaxin-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MPIF2, SCYA24, C-C motif chemokine 24, CK-beta-6, MPIF-2, Ckb-6 |
| Immunogen | The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human Eotaxin-2 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with our biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western blot: To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL24 |
|---|---|
| Alternative Names | MPIF2 SCYA24 C-C motif chemokine 24 CK-beta-6 MPIF-2 Ckb-6 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren
Application
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
Application
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
Application
To a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with ProSci's biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with our biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western blot: To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Eotaxin-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
