| Product Name | Eotaxin-3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCYA26, UNQ216/PRO242, C-C motif chemokine 26, CC chemokine IMAC, MIP-4-alpha, IMAC, TSC-1, MIP-4a, MIP-4alpha |
| Immunogen | Eotaxin-3 antibody is produced in BALB/c ICRR F1 mice using highly pure (>98%) recombinant human Eotaxin-3 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL26 |
|---|---|
| Alternative Names | SCYA26 UNQ216/PRO242 C-C motif chemokine 26 CC chemokine IMAC MIP-4-alpha IMAC TSC-1 MIP-4a MIP-4alpha |
| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Eotaxin-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
