Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK (Ab-861).| Product Name | FAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Shi Q, et al. (2003) Mol Biol Cell; 14 (10): 4306-15. Vadlamudi RK, et al. (2003) FEBS Lett; 543 (1-3): 76-80. Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157 (1): 149-60. Abu-Ghazaleh R, (2001) et al. Biochem J; 360 (Pt 1): 255-64. Slack JK, et al. (2001) Oncogene; 20 (10): 1152-63. |
| Immunogen | FAK (Ab-861) antibody was raised against a peptide sequence around aa. 859~863 (H-I-Y-Q-P) derived from Human FAK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | FAK FADK FAK1 FRNK PPP1R71 p125FAK pp125FAK KIAA0288 FADK 1 PTK2 |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | This antibody detects endogenous level of total FAK protein. |
| Entrez Gene | 5747 |
|---|---|
| Protein Accession | NP_005598.3 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK (Ab-861).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
