Application
Western blot analysis of lysed extracts from HeLa cells using FAK (Ab-925).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK (Ab-925). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using FAK (Ab-925).| Product Name | FAK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Sanders MA, et al. (2005) J Biol Chem; 280 (25): 23516-22. Cherubini A, et al. (2005) Mol Biol Cell; 16 (6): 2972-83. Toriumi Y, et al. (2003) FEBS Lett; 553 (3): 419-22. Shi Q, et al. (2003) Mol Biol Cell; 14 (10): 4306-15. |
| Immunogen | FAK (Ab-925) antibody was raised against a peptide sequence around aa.923~927 (K-V-Y-E-N) derived from Human FAK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | FAK FADK FAK1 FRNK PPP1R71 p125FAK pp125FAK Grasp1 FADK 1 PTK2 |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | This antibody detects endogenous level of total FAK protein. |
| Entrez Gene | 5747 |
|---|---|
| Protein Accession | NP_005598.3 |
Application
Western blot analysis of lysed extracts from HeLa cells using FAK (Ab-925).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK (Ab-925). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using FAK (Ab-925).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
