Application
Western blot analysis of extracts from HepG2 cells treated with PMA using Phospho-FAK (Ser843) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | FAK (phospho Ser843) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Jiang X, Sinnett-Smith J, Rozengurt E (2007)Cell Signal 19, 1000-10 . Jacamo R, Jiang X, Lunn JA, Rozengurt E (2007)J Cell Physiol 210, 436-44. Le Boeuf F, Houle F, Sussman M, Huot J (2006)Mol Biol Cell 17, 3508-20 . |
| Immunogen | FAK (phospho Ser843) antibody was raised against a peptide sequence around phosphorylation site of serine843 (R-G-S (p)-I-D) derived from Human FAK . |
| Modification | p-Ser843 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | FADK 1 FAK1 Focal adhesion kinase 1 PTK2 pp125FAK |
| Molecular Weight(MW) | 120 kDa |
| Tissue Specificity | The antibody detects endogenous level of FAK only when phosphorylated at serine 843. |
| Entrez Gene | 5747 |
|---|---|
| Protein Accession | NP_001186578.1 |
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using Phospho-FAK (Ser843) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
