Application
Western blot analysis of extract from 293 cells untreated or treated with EGF using FAK (Phospho-Tyr397) Antibody.| Product Name | FAK (phospho Tyr397) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Matsuya M. et. al. (1998) J. Biol. Chem. 273:1003-1014Fujita H. et. al. (1998) J. Biol. Chem. 273:26516-26521Le Romancer M. et. al. (2008) Mol. Cell 31:212-221 |
| Immunogen | FAK (Phospho-Tyr397) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 397 (D-D-Y (p) -A-E) derived from Human FAK. |
| Modification | p-Tyr397 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | FAK FADK FAK1 FRNK PPP1R71 p125FAK pp125FAK FAK PTK2 |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | This antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397. |
| Entrez Gene | 5747 |
|---|---|
| Protein Accession | NP_001186578.1 |
Application
Western blot analysis of extract from 293 cells untreated or treated with EGF using FAK (Phospho-Tyr397) Antibody.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
