Application
Immunofluorescence staining of methanol-fixed HeLa cells using FAK (phospho-Tyr576/Tyr577).Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577).| Product Name | FAK (phospho Tyr576 / Tyr577) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FAK, FADK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, EIF4F, EIF4G, EIF4GI, FADK 1, PTK2 |
| Product description | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Parsons, J. T. et al. (2000) Oncogene 19, 5606-5613Schaller, M. D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B. S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H. C. et al. (1996) J. Biol. Chem. 271, 26329-26334. |
| Immunogen | FAK (phospho-Tyr576/Tyr577) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y (p) -Y (p) -K-A) derived from Human FAK. |
| Modification | p-Tyr576 / Tyr577 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | FAK FADK FAK1 FRNK PPP1R71 p125FAK pp125FAK EIF4F EIF4G EIF4GI FADK 1 PTK2 |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | This antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577. |
| Entrez Gene | 5747 |
|---|---|
| Protein Accession | NP_005598.3 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using FAK (phospho-Tyr576/Tyr577).
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
