Application
Western blot analysis of extracts from NIH-3T3 tissue using FAK (Phospho-Tyr576) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | FAK (phospho Tyr576) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.1) Tong L, et al. (2013)Biochim Biophys Acta 1833, 1304-15. Chen S, et al. (2013)Sci Signal 6, ra40. Tian H, et al. (2012)EMBO J 31, 3885-900. |
| Immunogen | FAK (phospho Tyr576) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine576 (S-T-Y (p)-Y-K) derived from Human FAK. |
| Modification | p-Tyr576 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTK2 |
|---|---|
| Alternative Names | FADK 1 FAK1 Focal adhesion kinase 1 PTK2 pp125FAK |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576. |
| Entrez Gene | 5747 |
|---|---|
| Protein Accession | NP_001186578.1 |
Application
Western blot analysis of extracts from NIH-3T3 tissue using FAK (Phospho-Tyr576) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
