| Product Name | Fas Antibody [APO-1-3] (preservative free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-kappaB. |
| Immunogen | Recombinant human Fas. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Host Species | Mouse |
| Tested Applications | FACSFunctionalStudiesIPWB |
| ELISA: (direct or indirect: 1:2,000-1:10,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAS |
|---|---|
| Alternative Names | CD95 APO-1 TNFRSF6 Tumor Necrosis Factor Receptor Superfamily Member 6 Apoptosis-mediating Surface Antigen FAS |
| Tissue Specificity | Recognizes human Fas. |
| Entrez Gene | 355 |
|---|---|
| Protein Accession | NP_000034.1 |
| Application Notes | ELISA: (direct or indirect: 1:2,000-1:10,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. In PBS. Preservative free formulation. |
Data sheet for OM278555