LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may play an important role during the early stages of skeletal muscle differentiation, specifically in alpha5beta1-integrin-mediated signaling pathways.1) McGrath, M.J., et al., (2003) Am. J. Physiol., Cell Physiol. 285 (6), C1513-C1526.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FHL1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
FHL1 antibody can be used for detection of FHL1 by ELISA at 1:312500. FHL1 antibody can be used for detection of FHL1 by western blot at 0.1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
FHL1 antibody can be used for detection of FHL1 by ELISA at 1:312500. FHL1 antibody can be used for detection of FHL1 by western blot at 0.1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store FHL1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt