Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | FHL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FHL3 contains 4 LIM zinc-binding domains. It is expressed only in skeletal muscle. FHL3 association with MyoD may contribute to the regulation of MyoD-dependent transcription of muscle genes and thereby myogenesis.LIM proteins are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.1) Cottle, D.L., J. Cell. Sci. 120 (PT 8), 1423-1435 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FHL3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FHL3 antibody can be used for detection of FHL3 by ELISA at 1:1562500. FHL3 antibody can be used for detection of FHL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FHL3 |
|---|---|
| Alternative Names | FHL3 MGC19547 MGC23614 MGC8696 SLIM2 |
| Molecular Weight(MW) | 31 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | FHL3 antibody can be used for detection of FHL3 by ELISA at 1:1562500. FHL3 antibody can be used for detection of FHL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FHL3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
