Application
Western blot of HeLa lysate showing specific immunolabeling of the ~ 34k fibrillarin protein. Below: Human SH-SY5Y cells stained with mouse-anti-fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.| Product Name | Fibrillarin Antibody [38F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).1) Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985). |
| Immunogen | Yeast nuclear preparations. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHC,WB |
| Species Reactivity | Drosophila,Human,Rat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | NOP1 |
|---|---|
| Alternative Names | LOT3 LOT3 |
| Molecular Weight(MW) | 34 |
| Sequence Similarities | ORF Names: D2870, Histone-glutamine methyltransferase, Nucleolar protein 1 |
| Tissue Specificity | Specific for the ~34kDa Fibrillarin /Nop1p protein. |
Application
Western blot of HeLa lysate showing specific immunolabeling of the ~ 34k fibrillarin protein. Below: Human SH-SY5Y cells stained with mouse-anti-fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.| Form | Liquid |
|---|---|
| Storage Instructions | Fibrillarin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
