Application
Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 μg/mL.Application
Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 μg/mL.Application
Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 μg/ml.| Product Name | FKBP12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1). FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2). FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5).1) Siekierka JJ, Hung SH, Poe M, et al. A cytosolic binding protein for the immunosuppressant FK506 has peptidyl-prolyl isomerase activity but is distinct from cyclophilin. Nature 1989; 341:755-7. |
| Immunogen | FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12. The immunogen is located within the first 50 amino acids of FKBP12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FKBP1A |
|---|---|
| Alternative Names | FKBP12 Antibody: FKBP1 PKC12 PKCI2 FKBP12 PPIASE FKBP-12 FKBP-1A FKBP1 Peptidyl-prolyl cis-trans isomerase FKBP1A 12 kDa FK506-binding protein PPIase FKBP1A |
| Molecular Weight(MW) | Predicted: 12 kDa Observed: 12 kDa |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
| Tissue Specificity | FKBP12 antibody is human, mouse and rat reactive. |
Application
Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 μg/mL.
Application
Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 μg/mL.
Application
Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | FKBP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide. |
