Application
Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.Application
Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.| Product Name | FLIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog |
| Product description | FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.1) Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521. |
| Immunogen | FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| FLIP antibody can be used for the detection of FLIP by Western blot at 1-2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFLAR |
|---|---|
| Alternative Names | FLIP Antibody: CASH FLIP MRIT CLARP FLAME Casper FLAME1 c-FLIP FLAME-1 I-FLICE c-FLIPL c-FLIPR c-FLIPS CASP8AP1 CASH CASP8 and FADD-like apoptosis regulator Caspase homolog |
| Molecular Weight(MW) | Predicted: 53 kDa Observed: 58 kDa |
| Sequence Similarities | 3) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only. |
Application
Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | FLIP antibody can be used for the detection of FLIP by Western blot at 1-2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
