Application
Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 μg/ml.Application
Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/mL.Application
Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.Application
Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 μg/mL.Application
Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 μg/ml.| Product Name | FLIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog |
| Product description | FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.1) Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521 |
| Immunogen | FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants. The immunogen is located within the first 50 amino acids of FLIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICCIFIHC-PWB |
| FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFLAR |
|---|---|
| Alternative Names | FLIP Antibody: CASH FLIP MRIT CLARP FLAME Casper FLAME1 c-FLIP FLAME-1 I-FLICE c-FLIPL c-FLIPR c-FLIPS CASP8AP1 CASH CASP8 and FADD-like apoptosis regulator Caspase homolog |
| Molecular Weight(MW) | Predicted: 53 kDa Observed: 53 kDa |
| Sequence Similarities | 4) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | FLIP recognizes all FLIP splice variants including FLIPa, b, and y. |
Application
Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 μg/ml.
Application
Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/mL.
Application
Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.
Application
Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 μg/mL.
Application
Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | FLIP antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | FLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
