Application
Immunohistochemistry of FMR1 in human brain tissue with FMR1 antibody at 2.5 μg/mL.Application
Immunofluorescence of FMR1 in human brain tissue with FMR1 antibody at 20 μg/mL.Application
Western blot analysis of FMR1 in rat brain tissue lysate with FMR1 antibody at 1 μg/ml.| Product Name | FMR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FMR1 Antibody: Fragile X mental retardation protein 1, FMRP |
| Product description | Fragile X syndrome is a frequent form of inherited mental retardation caused by functional loss of the fragile X mental retardation protein, FMR1, also known as FMRP (1). FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm (2). A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome (1). Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1) (3).1) Jin P and Warren ST. Understanding the molecular basis of fragile X syndrome. Hum. Mol. Genet. 2000; 9:901-8. |
| Immunogen | FMR1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FMR1. The immunogen is located within the last 50 amino acids of FMR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| FMR1 antibody can be used for detection of FMR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FMR1 |
|---|---|
| Alternative Names | FMR1 Antibody: Fragile X mental retardation protein 1 FMRP |
| Molecular Weight(MW) | Predicted: 70 kDa Observed: 74 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | FMR1 antibody is human, mouse and rat reactive. Multiple isoforms of FMR1 are known to exist. |
Application
Immunohistochemistry of FMR1 in human brain tissue with FMR1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of FMR1 in human brain tissue with FMR1 antibody at 20 μg/mL.
Application
Western blot analysis of FMR1 in rat brain tissue lysate with FMR1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | FMR1 antibody can be used for detection of FMR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | FMR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | FMR1 antibody is supplied in PBS containing 0.02% sodium azide. |
