Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FXN antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of FXN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of FXN Antibody in CEM cell line lysates (35ug/lane)| Product Name | FXN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Frataxin, mitochondrial, Friedreich ataxia protein, Fxn, Frataxin intermediate form, i-FXN, Frataxin(56-210), m56-FXN, Frataxin(78-210), d-FXN, m78-FXN, Frataxin mature form, Frataxin(81-210), m81-FXN, FXN, FRDA, X25 |
| Product description | FXN is a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. |
| Immunogen | This FXN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-102 amino acids from the Central region of human FXN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | FXN |
|---|---|
| Alternative Names | Frataxin mitochondrial Friedreich ataxia protein Fxn Frataxin intermediate form i-FXN Frataxin(56-210) m56-FXN Frataxin(78-210) d-FXN m78-FXN Frataxin mature form Frataxin(81-210) m81-FXN FXN FRDA X25 |
| Molecular Weight(MW) | 23 kDa |
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FXN antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of FXN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of FXN Antibody in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
