Application
Western blot analysis of FXN Antibody (Center) in CEM cell line lysates (35ug/lane). FXN (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of FXN Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FXN antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Frataxin antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Frataxin (Internal)This nuclear gene encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 25~54 amino acids from the Center region of human Frataxin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | FXN FRDA Friedreich ataxia protein Frataxin intermediate form X25 Frataxin mitochondrial |
|---|---|
| Target | Frataxin |
| Molecular Weight(MW) | 23.135 kDa (note) |
| Cellular Localization | PubMed:18725397 reports localization exclusively in mitochondria. |
Application
Western blot analysis of FXN Antibody (Center) in CEM cell line lysates (35ug/lane). FXN (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of FXN Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with FXN antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
