Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
GAA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysates from A549, MCF-7, SW620, PC-3 cell line (from left to right), using GAA Antibody at 1:1000 at each lane.Application
Western blot analysis of lysate from Jurkat cell line, using GAA Antibody at 1:2000.Application
Antibody staining GAA in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | GAA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, 76 kDa lysosomal alpha-glucosidase, 70 kDa lysosomal alpha-glucosidase, GAA |
| Product description | This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This GAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-203 amino acids from the N-terminal region of human GAA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| For IHC starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAA |
|---|---|
| Alternative Names | Lysosomal alpha-glucosidase Acid maltase Aglucosidase alfa 76 kDa lysosomal alpha-glucosidase 70 kDa lysosomal alpha-glucosidase GAA |
| Molecular Weight(MW) | 105 kDa |
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
GAA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysates from A549, MCF-7, SW620, PC-3 cell line (from left to right), using GAA Antibody at 1:1000 at each lane.
Application
Western blot analysis of lysate from Jurkat cell line, using GAA Antibody at 1:2000.
Application
Antibody staining GAA in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
