Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
GAPDHS Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of GAPDHS Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | GAPDHS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, GAPDH-2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, GAPDHS, GAPD2, GAPDH2, GAPDS |
| Product description | GAPDHS is a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.1) Welch,J.E., et.al., J. Androl. 21 (2), 328-338 (2000) Goodwin,L.O., et.al., Mol. Hum. Reprod.(2), 127-136 (2000) Benham,F.J. et.al., Genomics(2), 209-214 (1989) |
| Immunogen | This GAPDHS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the Central region of human GAPDHS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GAPDHS |
|---|---|
| Alternative Names | Glyceraldehyde-3-phosphate dehydrogenase testis-specific Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 GAPDH-2 Spermatogenic glyceraldehyde-3-phosphate dehydrogenase GAPDHS GAPD2 GAPDH2 GAPDS |
| Molecular Weight(MW) | 45 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
GAPDHS Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of GAPDHS Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
