Application
Western blot of rat hippocampal lysate showing the immunolabeling of ~38k GAPDH protein. Below: Human neuroblastoma SH-SY5Y cells stained with mouse anti-GAPDH (green), chicken antibody to neurofilament NF-H (red) and DNA (blue).| Product Name | GAPDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GAPD2, GAPDS, HSD-35, GAPDH-2, GAPD2, GAPDH2, GAPDS |
| Product description | Biological Significance: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.1) Morgenegg G, Winkler GC, Hubscher U, Heizmann CW, Mous J, Kuenzle CC. Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. J Neurochem. 47:54-62 (1986). |
| Immunogen | Extensively purified pig GAPDH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | ChickenPorcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAPDHS |
|---|---|
| Alternative Names | GAPD2 GAPDS HSD-35 GAPDH-2 GAPD2 GAPDH2 GAPDS |
| Molecular Weight(MW) | 38 |
| Sequence Similarities | ORF Names: HSD-35, HSD35, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 |
| Tissue Specificity | Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein. |
Application
Western blot of rat hippocampal lysate showing the immunolabeling of ~38k GAPDH protein. Below: Human neuroblastoma SH-SY5Y cells stained with mouse anti-GAPDH (green), chicken antibody to neurofilament NF-H (red) and DNA (blue).| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GAPDH antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
