Trans-acting T-cell specific transcription factor GATA-3 is a member of GATA family of transcription factors that regulates development of multiple tissues. It is an important transcription factor in regulating human Th2 cell differentiation in vivo.1) Kaminuma, O., et al., (2004) FEBS Lett.570(1-3), 63-68.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
GATA3 antibody can be used for detection of GATA3 by ELISA at 1:312500. GATA3 antibody can be used for detection of GATA3 by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Liver at 4.0-8.0 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
GATA3 antibody can be used for detection of GATA3 by ELISA at 1:312500. GATA3 antibody can be used for detection of GATA3 by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GATA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.