Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.Application
Antibody used in WB on MCF7 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human kidney lysate at 5.0 ug/ml.| Product Name | GATA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GATA3, HDR, MGC2346, MGC5199, MGC5445, HDRS |
| Product description | GATA3 is crucially involved in IL-5 gene transcription in human peripheral CD4-positive t cells. This gene is involved in growth control and the maintenance of the differentiated state in epithelial cells and may contribute to tumorigenesis in breast cancer.1) Arshad, S.H., (2008) Br. J. Dermatol. 158 (6), 1315-1322. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GATA3 antibody can be used for detection of GATA3 by ELISA at 1:1562500. GATA3 antibody can be used for detection of GATA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA3 |
|---|---|
| Alternative Names | GATA3 HDR MGC2346 MGC5199 MGC5445 HDRS |
| Molecular Weight(MW) | 48 kDa |
Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.
Application
Antibody used in WB on MCF7 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney lysate at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | GATA3 antibody can be used for detection of GATA3 by ELISA at 1:1562500. GATA3 antibody can be used for detection of GATA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GATA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
