Application
Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 μg/mL.Application
Immunohistochemistry of GATA3 in mouse brain tissue with GATA3 antibody at 2.5 μg/ml.Application
Immunofluorescence of GATA3 in Rat Brain tissue with GATA3 antibody at 20 μg/mL.Application
Western blot analysis of GATA3 in K562 cell lysate with GATA3 antibody at 1 μg/mL.| Product Name | GATA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GATA3 Antibody: HDR, HDRS, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3 |
| Product description | GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.1) Ko LJ, Yamamoto M, Leonard MW, et al. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol. Cell. Biol. 1991; 11:2778-84. |
| Immunogen | GATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3. The immunogen is located within amino acids 340 - 390 of GATA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA3 |
|---|---|
| Alternative Names | GATA3 Antibody: HDR HDRS Trans-acting T-cell-specific transcription factor GATA-3 GATA-binding factor 3 |
| Molecular Weight(MW) | Predicted: 49 kDa Observed: 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Entrez Gene | 2625 |
|---|---|
| Protein Accession | NP_001002295 |
Application
Immunohistochemistry of GATA3 in rat brain tissue with GATA3 antibody at 2.5 μg/mL.
Application
Immunohistochemistry of GATA3 in mouse brain tissue with GATA3 antibody at 2.5 μg/ml.
Application
Immunofluorescence of GATA3 in Rat Brain tissue with GATA3 antibody at 20 μg/mL.
Application
Western blot analysis of GATA3 in K562 cell lysate with GATA3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GATA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GATA3 Antibody is supplied in PBS containing 0.02% sodium azide. |
