Application
Western blot analysis using GFP monoclonal antibody against full - length GFP recombinant protein and GFP transfected CHO cell lysate.| Product Name | GFP Antibody [4B10B2] |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.1) Dittman JS. Kaplan JM. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. Epub 2006 Jul 14. |
| Immunogen | Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | GFP |
| Concentration | 1mg/ml |
| Gene Symbol | gfp |
|---|---|
| Alternative Names | Green fluorescent protein |
| Entrez Gene | 7011691 |
|---|---|
| Protein Accession | YP_002302326.1 |
Application
Western blot analysis using GFP monoclonal antibody against full - length GFP recombinant protein and GFP transfected CHO cell lysate.| Application Notes | Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GFP monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
