Application
Western blot analysis of anti-GIT1 Antibody (Y510) in mouse cerebellum tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GIT1 Antibody (Y510) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | GIT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1 |
| Product description | GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.1) Sato,H., Biochem. Biophys. Res. Commun. 368 (1), 157-161 (2008) |
| Immunogen | This GIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-517 amino acids from human GIT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GIT1 |
|---|---|
| Alternative Names | ARF GTPase-activating protein GIT1 ARF GAP GIT1 Cool-associated and tyrosine-phosphorylated protein 1 CAT-1 CAT1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 GIT1 |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of anti-GIT1 Antibody (Y510) in mouse cerebellum tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GIT1 Antibody (Y510) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
