Application
GIT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | GIT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1 |
| Product description | GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.1) Sato,H., Biochem. Biophys. Res. Commun. 368 (1), 157-161 (2008) |
| Immunogen | This GIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 485-512 amino acids from the Central region of human GIT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GIT1 |
|---|---|
| Alternative Names | ARF GTPase-activating protein GIT1 ARF GAP GIT1 Cool-associated and tyrosine-phosphorylated protein 1 CAT-1 CAT1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 GIT1 |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
GIT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
