Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstratesdetected the GIT1 protein (arrow).Application
Western Blot at 1:1000 dilution Lane 1: U-2OS whole cell lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | GIT1-S388 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1 |
| Product description | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt).1) Kawachi, H., et al. Proc. Natl. Acad. Sci. U.S.A. 98(12):6593-6598(2001) Zhao, Z.S., et al. Mol. Cell. Biol. 20(17):6354-6363(2000) Premont, R.T., et al. J. Biol. Chem. 275(29):22373-22380(2000) Premont, R.T., et al. Proc. Natl. Acad. Sci. U.S.A. 95(24):14082-14087(1998) |
| Immunogen | This GIT1-S388 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the Central region of human GIT1-S388. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GIT1 |
|---|---|
| Alternative Names | ARF GTPase-activating protein GIT1 ARF GAP GIT1 Cool-associated and tyrosine-phosphorylated protein 1 CAT-1 CAT1 G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 GIT1 |
| Molecular Weight(MW) | 84 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstratesdetected the GIT1 protein (arrow).
Application
Western Blot at 1:1000 dilution Lane 1: U-2OS whole cell lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
