GJA3 belongs to the connexin family. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA3 are the cause of zonular pulverulent cataract type 3 (CZP3).1) Fritz, G.A., (2007) Rofo 179 (6), 637-638.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GJA3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GJA3 antibody can be used for detection of GJA3 by ELISA at 1:62500. GJA3 antibody can be used for detection of GJA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
GJA3 antibody can be used for detection of GJA3 by ELISA at 1:62500. GJA3 antibody can be used for detection of GJA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GJA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.