Application
Western blot analysis of GJA3 Antibody in Jurkat cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma;| Product Name | GJA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gap junction alpha-3 protein, Connexin-46, Cx46, GJA3 |
| Product description | GJA3, an integral membrane protein, belong to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin is a component of lens fiber gap junctions, can form both junctional and nonjunctional ('hemi-') channels. Defects in GJA3 are the cause of zonular pulverulent cataract type 3 (CZP3), a form of autosomal dominant congenital cataract.1) Mackay, D., et al., Am. J. Hum. Genet. 64(5):1357-1364 (1999). |
| Immunogen | This GJA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-139 amino acids from the N-terminal region of human GJA3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GJA3 |
|---|---|
| Alternative Names | Gap junction alpha-3 protein Connexin-46 Cx46 GJA3 |
| Molecular Weight(MW) | 47 kDa |
Application
Western blot analysis of GJA3 Antibody in Jurkat cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma;| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
