Application
Western blot of a rat hippocampal (Hipp) lysate showing the specific immunolabeling of the ~100k GluR2 protein.| Product Name | GluR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GluA2, GluR2, gluR-B, GluR-K2, Glur2, AMPA-selective glutamate receptor 2, GluR-2 |
| Product description | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990;Hollmann and Heinemann, 1994). The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD (Chung et al., 2003)1) Chung HJ, Steinberg JP, Huganir RL, Linden DJ (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755. |
| Immunogen | Peptide corresponding to amino acid residues from rat GluR2, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will also react with chicken, human, mouse, non-human primates and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gria2 |
|---|---|
| Alternative Names | GluA2 GluR2 gluR-B GluR-K2 Glur2 AMPA-selective glutamate receptor 2 GluR-2 |
| Molecular Weight(MW) | 100 |
Application
Western blot of a rat hippocampal (Hipp) lysate showing the specific immunolabeling of the ~100k GluR2 protein.| Application Notes | The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will also react with chicken, human, mouse, non-human primates and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GluR2 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
