Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR2/3 protein.| Product Name | GluR2/3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GluA2, GluR2, gluR-B, GluR-K2, Glur2, AMPA-selective glutamate receptor 2, GluR-2 |
| Product description | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990;Hollmann and Heinemann, 1994). The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory (Duprat et al., 2003; Seidenman et al., 2003; Chung et al., 2003; Yan et al., 2002).1) Chung HJ, Steinberg JP, Huganir RL, Linden DJ (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755. |
| Immunogen | Peptide corresponding to amino acid residues from the C-terminal region of rat GluR2/3 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will also react with chicken, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
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| Species Reactivity | ChickenHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gria2 |
|---|---|
| Alternative Names | GluA2 GluR2 gluR-B GluR-K2 Glur2 AMPA-selective glutamate receptor 2 GluR-2 |
| Molecular Weight(MW) | 100 |
Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR2/3 protein.| Application Notes | The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will also react with chicken, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GluR2/3 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
