Application
GNE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in human placenta tissue lysates (35ug/lane).| Product Name | GNE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase (hydrolyzing), UDP-GlcNAc-2-epimerase, Uridine diphosphate-N-acetylglucosamine-2-epimerase, N-acetylmannos |
| Product description | The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq].1) Stober, A., et al. Neuromuscul. Disord. 20(5):335-336(2010) |
| Immunogen | This GNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human GNE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNE |
|---|---|
| Alternative Names | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase UDP-GlcNAc-2-epimerase/ManAc kinase UDP-N-acetylglucosamine 2-epimerase (hydrolyzing) UDP-GlcNAc-2-epimerase Uridine diphosphate-N-acetylglucosamine-2-epimerase N-acetylmannos |
| Molecular Weight(MW) | 79 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Hamster |
Application
GNE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in human placenta tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
