Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected GNE protein (arrow).| Product Name | GNE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase (hydrolyzing), UDP-GlcNAc-2-epimerase, Uridine diphosphate-N-acetylglucosamine-2-epimerase, N-acetylmannos |
| Product description | GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.1) Reinke, S.O., et al. Glycoconj. J. 26(4):415-422(2009) |
| Immunogen | This GNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 580-607 amino acids from the C-terminal region of human GNE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNE |
|---|---|
| Alternative Names | Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase UDP-GlcNAc-2-epimerase/ManAc kinase UDP-N-acetylglucosamine 2-epimerase (hydrolyzing) UDP-GlcNAc-2-epimerase Uridine diphosphate-N-acetylglucosamine-2-epimerase N-acetylmannos |
| Molecular Weight(MW) | 79 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Hamster |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected GNE protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
