Application
Western blot analysis using GST monoclonal antibody against GST recombinant protein.| Product Name | GST Antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | gst, b1635, JW1627, GST B1-1 |
| Product description | Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.1) Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40. |
| Immunogen | Ni-NTA purified recombinant GST expressed in E. Coli strain M15. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | GST |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | gst |
|---|---|
| Alternative Names | gst b1635 JW1627 GST B1-1 |
Application
Western blot analysis using GST monoclonal antibody against GST recombinant protein.| Application Notes | Western Blot:Dilution 1:2000 - 5,000 ELISA:Proposed dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GST monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
