Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Product Name | GTF2E1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTF2E1, FE, TF2E1, TFIIE-A |
| Product description | GTF2E1 belongs to the TFIIE alpha subunit family. It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.1) Okuda, M., (2008) EMBO J. 27 (7), 1161-1171. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:312500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF2E1 |
|---|---|
| Alternative Names | GTF2E1 FE TF2E1 TFIIE-A |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:312500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTF2E1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
