Application
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Muscle.| Product Name | GTF2E1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTF2E1, FE, TF2E1, TFIIE-A |
| Product description | The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation1) Ohkuma, Y., et al., (1991) Nature 354 (6352), 398-401. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF2E1 |
|---|---|
| Alternative Names | GTF2E1 FE TF2E1 TFIIE-A |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Muscle.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTF2E1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
