Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.Application
Antibody used in WB on transformed bacteria at: 1:1000 (Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate, Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample).| Product Name | HCFC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HCFC1 is a member of the host cell factor family with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection.1) Director MGC Project, (2003) Direct Submission. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCFC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HCFC1 antibody can be used for detection of HCFC1 by ELISA at 1:312500. HCFC1 antibody can be used for detection of HCFC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HCFC1 |
|---|---|
| Alternative Names | HCFC1 CFF HCF1 HFC1 MRX3 VCAF HCF-1 |
| Molecular Weight(MW) | 45 kDa, 209 kDa |
Application
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.
Application
Antibody used in WB on transformed bacteria at: 1:1000 (Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate, Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | HCFC1 antibody can be used for detection of HCFC1 by ELISA at 1:312500. HCFC1 antibody can be used for detection of HCFC1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HCFC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
