WB
Various lysates were subjected to SDS PAGE followed by western blot with HCFC1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using HCFC1 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0)ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).IP
IP result of anti-HCFC1 with HeLa cells lysate 1600 ug.| Product Name | HCFC1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human HCFC1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIPWB |
| WB:1:1000-1:8000 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:1000-1:4000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HCFC1 |
|---|---|
| Gene Synonyms | CFF HCF HCF1 HFC1 MRX3 VCAF HCF-1 MAHCX XLID3 PPP1R89 |
| Gene Full Name | host cell factor C1 |
| Gene Summary | This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | HCF C-terminal chain 2 HCF C-terminal chain 1 HCF 1 HCF CFF |
| Molecular Weight(MW) | 300 kDa(Observed band size:209 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with HCFC1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using HCFC1 antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0)
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IP
IP result of anti-HCFC1 with HeLa cells lysate 1600 ug.| Application Notes | WB:1:1000-1:8000 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:1000-1:4000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
