WB
Western blot analysis of HCFC1 using anti-HCFC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HCFC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody . HCFC1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody. HCFC1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody. HCFC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody. HCFC1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of HCFC1 using anti-HCFC1 antibody and anti-Beta Tubulin antibody. HCFC1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IP
IP analysis of HCFC1 using anti-HCFC1 antibody in K562 whole cell lysate. Western blot analysis of HCFC1 using anti- HCFC1 antibody. Lane 1: K562 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- HCFC1 antibody in K562 whole cell lysate, Lane 3: anti- HCFC1 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HCFC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of HepG2 cells using anti-HCFC1 antibody. Overlay histogram showing HepG2 cells stained with anti-HCFC1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCFC1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to HCFC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human HCFC1 recombinant protein (Position: R1204-Q2035). Human HCFC1 shares 90.2% and 89.7% amino acid (aa) sequence identity with mouse and rat HCFC1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:200-1:2000 IHC:1:100-1:1000 ICC/IF:1:50-1:200 IP:1:10-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HCFC1 |
|---|---|
| Gene Synonyms | CFF HCF HCF1 HFC1 MRX3 VCAF HCF-1 MAHCX XLID3 PPP1R89 |
| Gene Full Name | host cell factor C1 |
| Gene Summary | This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | C1 factor CFF HCF HCF 1 HCF1 HCFC1 HFC1 Host cell factor 1 VCAF VP16 accessory protein |
| Molecular Weight(MW) | 209 kDa(Observed band size: 130 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of HCFC1 using anti-HCFC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HCFC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody . HCFC1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody. HCFC1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody. HCFC1 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HCFC1 using anti-HCFC1 antibody. HCFC1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of HCFC1 using anti-HCFC1 antibody and anti-Beta Tubulin antibody. HCFC1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-HCFC1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IP
IP analysis of HCFC1 using anti-HCFC1 antibody in K562 whole cell lysate. Western blot analysis of HCFC1 using anti- HCFC1 antibody. Lane 1: K562 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- HCFC1 antibody in K562 whole cell lysate, Lane 3: anti- HCFC1 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- HCFC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of HepG2 cells using anti-HCFC1 antibody. Overlay histogram showing HepG2 cells stained with anti-HCFC1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCFC1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 ICC/IF:1:50-1:200 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
