Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Ab-394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using HDAC2 (Ab-394).Application
Western blot analysis of lysed extracts from 293 and HeLa cells using HDAC2 (Ab-394).| Product Name | HDAC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes1) Tsai SC, et al. (2002) J Biol Chem; 277 (35): 31826-33 |
| Immunogen | HDAC2 (Ab-394) antibody was raised against a peptide sequence around aa. 392~396 (E-D-S-G-D) derived from Human HDAC2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC2 |
|---|---|
| Alternative Names | HD2 RPD3 YAF1 SVP65 SYT HD2 Histone deacetylase 2 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of total HDAC2 protein. |
| Entrez Gene | 3066 |
|---|---|
| Protein Accession | NP_001518.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Ab-394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using HDAC2 (Ab-394).
Application
Western blot analysis of lysed extracts from 293 and HeLa cells using HDAC2 (Ab-394).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
