Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394).| Product Name | HDAC2 (phospho Ser394) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes1) Tsai SC, et al. (2002) J Biol Chem; 277 (35): 31826-33 |
| Immunogen | HDAC2 (Phospho-Ser394) antibody was raised against a peptide sequence around phosphorylation site of serine 394 (E-D-S (p) -G-D) derived from Human HDAC2. |
| Modification | p-Ser394 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC2 |
|---|---|
| Alternative Names | HD2 RPD3 YAF1 PRKCT HD2 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of HDAC2 only when phosphorylated at serine 394. |
| Entrez Gene | 3066 |
|---|---|
| Protein Accession | NP_001518.2 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
