Application
Antibody used in IHC on Human Spermatophore.Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | HDAC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.1) Choi, Y.B., et al., (2004) J.Biol.Chem.279(49), 50930-50941. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| HDAC2 antibody can be used for detection of HDAC2 by ELISA at 1:12500. HDAC2 antibody can be used for detection of HDAC2 by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC2 |
|---|---|
| Alternative Names | HDAC2 HD2 RPD3 YAF1 |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in IHC on Human Spermatophore.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HDAC2 antibody can be used for detection of HDAC2 by ELISA at 1:12500. HDAC2 antibody can be used for detection of HDAC2 by western blot at 0.2-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HDAC2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
