Application
Western blot analysis of hHDAC7-A935 in CEM cell line lysates (35ug/lane)| Product Name | HDAC7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.1) Meinke PT and Liberator P. Curr Med Chem, 8(2): 211- 235 (2001). |
| Immunogen | This HDAC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 920-952 amino acids from the C-terminal region of human HDAC7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | HDAC7 |
|---|---|
| Alternative Names | Histone deacetylase 7 HD7 Histone deacetylase 7A HD7a HDAC7 HDAC7A |
| Molecular Weight(MW) | 103 kDa |
Application
Western blot analysis of hHDAC7-A935 in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
