Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HEXA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HEXA Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | HEXA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases.1) Park,N.J., et.al., Pediatr. Res. (2009) |
| Immunogen | This HEXA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-343 amino acids from the Central region of human HEXA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HEXA |
|---|---|
| Alternative Names | Beta-hexosaminidase subunit alpha Beta-N-acetylhexosaminidase subunit alpha Hexosaminidase subunit A N-acetyl-beta-glucosaminidase subunit alpha HEXA |
| Molecular Weight(MW) | 61 kDa |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HEXA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HEXA Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
