Application
Western blot analysis of HFE antibody in Jurkat cell line lysates (35ug/lane)Application
HFE Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | HFE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in its gene.1) Valenti,L., ClinAm Soc Nephrol(8), 1331-1337 (2009) |
| Immunogen | This HFE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-148 amino acids from the Central region of human HFE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | HFE |
|---|---|
| Alternative Names | Hereditary hemochromatosis protein HLA-H HFE HLAH |
| Molecular Weight(MW) | 40 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of HFE antibody in Jurkat cell line lysates (35ug/lane)
Application
HFE Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
