Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Antibody is used in Western blot to detect HGF in Ramos tissue lysate. HGF (arrow) was detected using the purified Pab.| Product Name | HGF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.1) Ryugo, M., et al., Transplantation 78(8):1153-1158 (2004). |
| Immunogen | This HGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from the C-terminal region of human HGF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | HGF |
|---|---|
| Alternative Names | Hepatocyte growth factor Hepatopoietin-A Scatter factor SF Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain HGF HPTA |
| Molecular Weight(MW) | 83 kDa |
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Antibody is used in Western blot to detect HGF in Ramos tissue lysate. HGF (arrow) was detected using the purified Pab.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
