WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HGF antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human liver was stained with anti-HGF Rabbit antibody.| Product Name | HGF Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:200-1:1000 WB:1:1000-1:5000 IF:1:200-1:1000 ELISA:1:5000-1:20000 IP:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HGF |
|---|---|
| Gene Synonyms | SF HGFB HPTA F-TCF DFNB39 |
| Gene Full Name | hepatocyte growth factor |
| Gene Summary | This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015] |
| Alternative Names | HGF HPTA Hepatocyte growth factor Hepatopoietin-A Scatter factor SF |
| Molecular Weight(MW) | 83 kDa (Observed band size: 85kDa) |
| Cellular Localization | extracellular region ,extracellular space ,membrane ,platelet alpha granule lumen , |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HGF antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human liver was stained with anti-HGF Rabbit antibody.| Application Notes | IHC:1:200-1:1000 WB:1:1000-1:5000 IF:1:200-1:1000 ELISA:1:5000-1:20000 IP:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
