Immunohistochemistry
Immunohistochemistry with Human Adrenal Gland lysate tissue at an antibody concentration of 5.0ug/ml using anti-HGF antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Product Name | Rabbit anti-HGF polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | F-TCF, HGFB, HPTA, SF, DFNB39 |
| Immunogen | The immunogen for anti-HGF antibody: synthetic peptide directed towards the N terminal of human HGF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMouseRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HGF |
|---|---|
| Gene Synonyms | F-TCF HGFB HPTA SF DFNB39 |
| Gene Full Name | Hepatocyte growth factor (hepapoietin A; scatter factor) |
| Gene Summary | Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity.Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. |
| Alternative Names | F-TCF HGFB HPTA SF DFNB39 |
| Molecular Weight(MW) | 79kDa |
| Sequence | 723 amino acids |
| Entrez Gene | 3082 |
|---|---|
| SwissProt ID | P14210 |
| Protein Accession | NP_001010932 |
Immunohistochemistry
Immunohistochemistry with Human Adrenal Gland lysate tissue at an antibody concentration of 5.0ug/ml using anti-HGF antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
